Journal: Nature cell biology

Article Title: Cell-matrix signals specify bone endothelial cells during developmental osteogenesis

doi: 10.1038/ncb3476

Figure Lengend Snippet: a , Immunostaining for Emcn (red) and the type E markers Caveolin 1 (green) and BCAM (white) in P6 femur. Panels on the right show higher magnification of corresponding insets. b , c , Transverse sections through P6 femur showing CD31 (green) and Caveolin 1-positive (white) vessels in compact bone (cb) (arrowheads) ( b ). By contrast, the bone marrow cavity (mc) contains Emcn-positive vessels with low CD31 signal (arrow). BCAM-positive vessels (white) in compact bone were associated with Osterix-expressing osteoprogenitors (green) ( c ). d , Quantitation of Osterix positive cells in proximity to type L vessels in the marrow cavity, metaphyseal type H vessels and to type E vessels in compact bone normalized to vessel length. Data represents mean± s.e.m. (n=6 mice), (p<0.001 between all compared groups, two-tailed unpaired t-test). e , RNA-seq-based expression levels of the indicated transcripts relative to expression in type L in freshly isolated P6 EC subpopulations. Data based on RPKM values obtained from RNA-seq. Data represents mean± s.e.m. (n=3 independent experiments). Statistics source data are shown in . f , Transcript expression in EC-C3H10T1/2 spheroid co-cultures (at day 7). Transcripts encoding Sox9, Runx2, Osteopontin ( Sp7 ), integrin binding sialoprotein ( Ibsp ), and Osteocalcin ( Bglap ) were upregulated in cultures containing primary type H or type E ECs. Data represents mean± s.e.m. (n=7 independent experiments), two-tailed unpaired t-test. g , Immunostaining of EC-C3H10T1/2 spheroids after 7 days in culture. Signals for Runx2 (white), Osteocalcin (green) and Alkaline phosphatase (red) were upregulated by CD31 hi (green) type H and type E ECs (arrowheads) but not in the presence of type L ECs. Nuclei, DAPI (blue). ECs were pre-labeled by DiI (red) at the onset of the experiments.

Article Snippet: The following FAM-conjugated TaqMan gene expression probes were used in combination with TaqMan Gene Expression Master Mix (Life Technologies): Flt4 (Mm00433337_m1), Pecam1 (Mm01242584_m1), Efnb2 (Mm01215897_m1), Notch1 (Mm00435245_m1), Notch3 (Mm01345646_m1), Bcam (Mm01321454_g1), Itgb1 (Mm01253233_m1), Esm1 (MM00469953_M1), Kitl (Mm00442972_m1), Unc5b (Mm00504054_m1), Cav1 (Mm00483057_m1), Itga5 (Mm00439797_m1), Itgav (Mm00434506_m1), Lama4 (Mm01193660_m1), Lama5 (Mm01222051_g1), Pdgfa (Mm01205760_m1), Pdgfb (Mm01298578_m1), Tgfb1 (Mm01178820_m1), Tgfb3 (Mm00436960_m1), Bmp2 (Mm01340178_m1), Bmp4 (Mm00432087_m1), Fgf1 (Mm00438906_m1), Dll4 (Mm00444619_m1), Noggin (Mm01297833_s1), Sox9 (Mm00448840_m1), Runx2 (Mm00501584_m1), Sp7 (Mm04209856_m1), Ibsp (Mm00492555_m1), Bglap (Mm03413826_mH).

Techniques: Immunostaining, Expressing, Quantitation Assay, Two Tailed Test, RNA Sequencing, Isolation, Binding Assay, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Argania Spinosa Fruit Shell Extract-Induced Melanogenesis via cAMP Signaling Pathway Activation

doi: 10.3390/ijms21072539

Figure Lengend Snippet: Effect of argan fruit shell ethanol extract (AFSEE) on mRNA expression of CREB binding protein ( Crebbp ), paired box gene 3 ( Pax3 ), lymphoid enhancer binding factor 1 ( Lef1 ), and SRY box-containing gene 10 ( Sox 10 ). B16F10 cells were cultured in a 100 mm dish at a density of 3 × 10 5 cells/dish and treated without (control) or with α-MSH (200 mM) or AFSEE (6 µg/mL and 30 µg/mL) for 1 h and 4 h ( A ) The effect of AFSEE on Crebbp , ( B ) Pax3 , ( C ) Lef1 , and ( D ) Sox10 expression was quantified using TaqMan real-time PCR. Data were subjected to ANOVA ( n = 4). All comparisons were made between treatments. Different letters indicate treatment differences at the p ≤ 0.05 level.

Article Snippet: Real-time Primers for Mitf (Mm0043495-m1), Tyr (Mm00495817-m1), Trp1 (Mm00453201-m1), Dct (Mm01225584-m1), Crebbp (Mm01342452-m1), Pax3 (Mm00435491-m1), Lef1 (Mm00550265-m1), and Sox10 (Mm00569909-m1) were used (Applied Biosystems, Foster City, CA, United States).

Techniques: Expressing, Binding Assay, Cell Culture, Control, Real-time Polymerase Chain Reaction

Journal: Bioengineering & Translational Medicine

Article Title: The combination of resveratrol and Bletilla striata polysaccharide decreases inflammatory markers of early osteoarthritis knee and the preliminary results on LPS ‐induced OA rats

doi: 10.1002/btm2.10431

Figure Lengend Snippet: Gene expression of chondrocyte; those were normalized to housekeeping gene (GAPDH) and control as ΔΔCt. (a) iNOS (b) IL‐1β, (c) IL‐6, (d) MMP13, (e) TGF‐β1, (f) SOX9, and (g) type II collagen. n = 6, * p < 0.05 compared with LPS group.

Article Snippet: The target genes of the RT‐qPCR experiment were IL‐1β (Ss03393804_m1), IL‐6 (Ss03384604_u1), MMP13 (Ss03373279_m1), iNOS (Ss03374608_u1), TGF‐β1 (Ss03382325_u1), SOX9(Ss03392406_m1), and type II collagen (Ss03373342_g1).

Techniques: Gene Expression, Control

Journal: Cells

Article Title: Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro

doi: 10.3390/cells10082126

Figure Lengend Snippet: List of primers used for quantitative PCR analysis.

Article Snippet: SOX2 , Sus scrofa , SRY-Box Transcription Factor 2 , Ss03388002_u1.

Techniques: Real-time Polymerase Chain Reaction

Journal: Cells

Article Title: Ovarian Decellularized Bioscaffolds Provide an Optimal Microenvironment for Cell Growth and Differentiation In Vitro

doi: 10.3390/cells10082126

Figure Lengend Snippet: Gene expression changes in pOCs (line bars), pEpiE (white with black dots bars), and hEpiE (black with white dots bars) during the ECM-based scaffold repopulation process. Expression pattern of fibroblast-specific (VIM, and THY1), pluripotency-related (OCT4, NANONG, REX1, and SOX2), and granulosa-cell-associated (STAR, CYP11A1, CYP19A1, AMH, FSHR, and LHCGR) markers in freshly isolated pOCs and porcine and human untreated fibroblasts (T0), in fibroblasts exposed to 5-aza-CR (post 5- aza-CR), and at different time points of ECM-based scaffold repopulation (24 and 48 h and 7 days). (a), (b), and (c) indicate statistically significant differences ( p < 0.05).

Article Snippet: SOX2 , Sus scrofa , SRY-Box Transcription Factor 2 , Ss03388002_u1.

Techniques: Gene Expression, Expressing, Isolation

Journal: Bone

Article Title: Pharmacological TNAP inhibition efficiently inhibits arterial media calcification in a warfarin rat model but deserves careful consideration of potential physiological bone formation/mineralization impairment

doi: 10.1016/j.bone.2020.115392

Figure Lengend Snippet: The mRNA expression of osteo/chondrogenic marker genes and genes involved in pyrophosphate/inorganic phosphate balance.

Article Snippet: Quantitative real time PCR The mRNA transcript expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Rn99999916_s1), TNAP (Rn01516028_m1), Runt-related transcription factor 2 (Runx2) (Rn01512296_m1), collagen 1 (Rn00801649_g1), collagen 2 (Rn00491926_g1) and SRY-box transcription factor 9 (Sox9) (Rn01751069_mH), matrix Gla protein (MGP) (Rn00563463_m1), ectonucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) (Rn01638706_m1) and 3 (NPP3) (Rn00571329_m1) and phosphoethanolamine/phosphocholine phosphatase 1 (PHOSPHO1) (Rn01496968_m1) was determined in the distal part of the abdominal aorta.

Techniques: Expressing, Marker, Gene Expression